Bosterbio
Anti-CD44 antibody
PA1021-2
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Browse Similar Products: CD44 Western Blot Antibodies
I used this anti-CD44 C-terminus antibody to detect cleaved/non-cleaved CD44 product. The results for Western blot are okay. The signal (1:1500) is not super strong but good enough to see clear bands. This antibody could detect a band the same size as another antibody that I used against the extracellular region of CD44, indicating this antibody could detect CD44.
Western Blot
Human breast cancer cell line
1:1500, 4 degrees celcius, O/N
5%BSA in TBST
Goat anti-rabbit HRP, 1:500, 1h at RT
Thermo super signal femto
Bio-Rad imager
The WB result showed multiple bands, some of the bands I am interested in overlapped with the bands generated by another CD44 antibody targeting the extracellular domains. So, the specificity of this antibody is probably okay. The intensity of bands are acceptable, good enough for publishing.
CD44 has multiple spliced isoforms
Relatively inexpnsive, okay specificity
Intensity of bands was at the low end
Good enough to detect CD44 C-term