P-ERK Used for Western Blot

pathology
upmc
research associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Cell Signaling Technology

Catalog Number:

4370S

I do cloning for animal experiments to use as liver cancer models and thus use a variety of molecular biology reagents including antibodies, plasmids, and RNA kits. With this antibody, 1:1000 dilution was used for Western blot, and we only needed to follow the general Western blot protocol.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Protein lysis

Primary Incubation

Just follow the Western blot protocol, 1:1000 dilution, 4 degrees overnight

Blocking Agent

5% BSA

Secondary Incubation

1:10000 in washing buffer 1hour at room temperature

Tertiary Incubation

NA

Detection

ECL

Results Summary

It's a very good antibody.

Additional Notes

None

Related Categories

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Summary

The Good

Good antibody

The Bad

None

The Bottom Line

No additional requirement

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