Mouse Skin CD3 Lymphocytes

December 29, 2015

UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

SouthernBiotech

Product Name:

Rat anti-mouse CD3e

Catalog Number:

1535-01

Our goal is to detect CD3 positive lymphocytes in mouse skin wounds. The staining with this antibody is okay with some background.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse skin

Primary Incubation

1/400 dilution, 45 min RT

Blocking Agent

Goat serum

Secondary Incubation

Alexa Fluor 594 goat anti rat

Tertiary Incubation

NA

Detection

Alexa Fluor 594

Results Summary

The staining is OK with some background.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to work with, good staining, not excellent

The Bad

Some background

The Bottom Line

Recommended

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