A Powerful Inverted Fluorescent Microscope for Any Task

Rice University
BioEngineering
Researcher

Overall

Performance

Ease-of-Optimization

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Company:

Nikon

Product Name:

ECLIPSE Ti

Catalog Number:

Ti-E

The Nikon Eclipse Ti inverted fluorescent microscope serves our lab's fluorescent imaging needs, from simple IF assays to completely automated time-lapse imaging of cells in microwell plates.

Experimental Design and Results Summary

Application

Fluorescent microscopy

Starting Material

Human Osteoblasts

Protocol Overview

Fluorescent microscopy to evaluate collagen (red channel) deposition and nuclear location (blue channel) by primary human osteoblasts. 20x objective.

Tips

Ensure co-witness between eyepieces, camera, and different objectives.

Results Summary

Imaging showed osteoblasts depositing collagen as part of ECM formation.

Features Summary

Incredibly powerful and modular fluorescent microscope. Automated and time-lapse features are very useful.

Additional Notes

None

Image Gallery

Summary

The Good

Crisp, publication-quality images that you would expect from Nikon optics.

The Bad

Cost. Acquisition and analysis software isn't the most intuitive.

The Bottom Line

The full Ti-E model may be overkill for basic fluorescent imaging applications, but as a unit it offers a powerful solution for multimode imaging with automation and time-lapse controls.

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