Rat Anti-Canine CD5 (FITC)

January 13, 2016

Vet. pathobiology
University of Missouri
scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Antibodies-online

Product Name:

Rat anti-canine CD5 (FITC) antibody

Catalog Number:

ABIN642155

The purpose of study was to compare natural killer cell populations between dogs with cancer and normal dogs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Blood from dogs

Primary Incubation

1:200 dilution, 30 min, on ice

Blocking Agent

FBS in PBS

Secondary Incubation

No need, since FITC conjugated

Tertiary Incubation

No need

Detection

Flow cytometry

Results Summary

There are no significant difference of NK cell population between dogs with cancer and normal dogs, but may have functional difference.

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use and time saving.

The Bad

Expensive

The Bottom Line

Time saving since no need for 2nd incubation and results show pretty clear separation from other cells.

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