Good Staining of CD4+ T Cells in Human Peripheral Blood

Pulmonary
Harvard Medical School
Post Doc

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Quality of Results

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Company:

BioLegend

Product Name:

APC anti-human CD4 Antibody

Catalog Number:

344613

The aim of my study was an immunophenotypic analysis of human peripheral blood leukocytes using minimal amounts of blood. Therefore I needed a good antibody that was reliable to identify CD4+ T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

50ul of human peripheral blood was stained with the antibody for 30 minutes at room temperature. Then sample was lysed with BD lysing solution according to the manufacture's protocol. Cells were washed three times with 1ml PBS and the resuspended in 300l FACs buffer and directly taken to flow cytometer

Primary Incubation

30 minutes

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This antibody identifies CD4+ T cells were reliable and clear.

Additional Notes

None

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Summary

The Good

Good antibody to identify CD4+ T cells

The Bad

None

The Bottom Line

If you want to identify Cd4+ T cells using flow cytometry this is a good product.

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