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Alexa Fluor® 488 anti-human CD4 Antibody
300519
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The aim of my study was a phenotypic analysis of major leukocyte subsets in human peripheral blood.
Flow Cytometry
Human peripheral blood
50ul of blood was incubated with the antibody for 30 minutes in the dark at RT. Cells were lysed with BD lysing solution following the instructions on the solution. Then cells were washed with PBS 3 times and then diluted in 300ul of FACS buffer for direct usage in flow cytometry.
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Flow cytometry
CD4+ T cells were clearly identified with this antibody. I can highly recommend using this antibody.
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Clear separation of CD4+ T cells in human peripheral blood
For immunophentotypic analysis of human CD4+ T cells this antibody provides a good tool.