BD Pharmingen
Purified Mouse Anti-Ki-67
550609
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Browse Similar Products: Ki-67 Immunofluorescence Antibodies
We were determining the proliferative capacity of beta cells in a transgenic mouse model utilized in our lab. Ki-67 is a nuclear cell proliferation-associated antigen expressed in all active stages of the cell cycle, and was suitable for these purposes.
Immunofluorescence
Mouse pancreatic paraffin-section
1:1000, 4 Deg C, overnight.
5% Normal Donkey Serum in 1%BSA/PBS (w/v)
1:1000. Mouse IgG-Cy2. 2 hours, Room temperature
NA
Fluorescence microscopy
This antibody performed as expected. Several insulin positive beta cells were found proliferating as observed by immunofluorescence co-staining. See Yellow arrowheads in supplied image.
Antigen retrieval (TEG, pH9.0) was necessary to detect Ki-67 in paraffin embedded sections.
Excellent detection at 1:1000
None
This antibody worked beautifully. Requires antigen retrieval if samples are embedded in paraffin.