Mouse Spleen CD8 Immunofluorescence Staining

UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

Purified rat anti mouse CD8a

Catalog Number:

550797

The aim was to detect CD8 cells in the spleen. This antibody did its job well. It was easy to use and less expensive.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse spleen

Primary Incubation

0.3125ug/ml, 45 min, RT

Blocking Agent

10% goat serum

Secondary Incubation

Alexa flour 488 goat anti rat

Tertiary Incubation

NA

Detection

Alexa flour 488

Results Summary

CD8 lymphocytes were well stained in the spleen

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use, less expensive, good signal

The Bad

Some background

The Bottom Line

Recommended

Share your experience with other scientists. Write a Review! »

Join the discussion