Mouse Skin Vimentin Immunofluorescence Staining

January 11, 2016

UIC
Research Scientist

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Company:

Abcam

Product Name:

Anti vimentin antibody

Catalog Number:

ab24525

This polyclonal chicken anti vimentin antibody works well to stain fibroblasts in mouse skin. There is less background compared to other tested antibodies.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse skin

Primary Incubation

45 min, 1/4500 dilution

Blocking Agent

10% goat serum

Secondary Incubation

45 min Goat Anti-Chicken (Texas Red)

Tertiary Incubation

NA

Detection

Texas Red

Results Summary

Mouse skin fibroblasts stained well.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

High dilution, use less antibody

The Bad

Some background

The Bottom Line

Recommended

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