R&D Systems
Human/Mouse GLI‑3 Antibody
AF3690
Info: View Product View Product Specs
Browse Similar Products: Gli3 Western blot Antibodies
The goal of this antibody was to test if Gli3 full length and repressor forms are expressed in NIH3T3 cells and if their levels change in response to Sonic Hedgehog ligand addition.
Western Blot
NIH3T3 cells
1:200 dilution in blocking buffer for 16 hr at 4 deg
5% Milk powder in 1xTBS-T (0.1% Tween)
1:5000 dilution (Donkey anti-goat IgG HRP) in blocking buffer for 1 hr at room temp
NA
ECL pico for 5 min
The antibody detects both both Gli3 full length (~200 kDa) and Gli3 repressor (~80 kDa) forms. After Sonic Hedgehog ligand addition for 24 hours, both Gli3 full length and repressor forms are degraded (as published in literature).
None
Detects both Gli3 full length and repressor forms
If you use ECL pico or ECL Femto for detection, you will get a good signal with this antibody