Abcam
Anti-Lamin A antibody
ab26300
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This experiment involved nuclear-cytoplasmic fractionation and Lamin A antibody was used to check the purity of the fractionation.
Western Blot
NIH3T3 cells
1:2000 dilution in blocking buffer for 16 hr at 4 deg
5% Milk in 1x TBS-T (0.1% Tween)
Donkey anti-Rabbit HRP antibody was used at 1:10000 dilution for 1 hr at room temp
NA
ECL for 10 sec on a film
NIH3T3 cells were fractionated into cytoplasmic and nuclear fractions. Lamin A was detected only in the nuclear fraction.
None
Specific signal at 75 kDa
Very good antibody for Western Blotting and it never failed!