Good Machine for Four Color Staining

University of California
Rheumatology
Graduate Student

Overall

Performance

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD FACS Calibur

Catalog Number:

BD FACSCalibur

FACS calibur was used to run the stained cells from mouse lymph nodes.

Experimental Design and Results Summary

Application

Flow cytometer analysis of mouse lymph node cells

Starting Material

Mouse lymph node preparation

Protocol Overview

Mouse lymph nodes were harvested. They were then mashed and stained with four colors FITC, PE, PE-Cy5 and APC

Tips

PE- Cy5 leaks extensively in FL2 and FL3 unless voltages are adjusted properly and dilution maintained

Results Summary

Results are better with just FL1, FL2 and FL4 lasers

Features Summary

Easy to use, also does not require too much instrument understanding

Additional Notes

None

Image Gallery

Summary

The Good

Easy to use and clean up

The Bad

Clogs fast and slow to run

The Bottom Line

Overall good if you want to do a quick two or three color staining procedure

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