Perfect Antibody for Total AKT Detection

January 08, 2016

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

AKT antibody

Catalog Number:

9272

I am investigating the role of the AKT-Pathway after extracellular vesicle treatment. I chose this product since I am also using the phosphor-AKT antibody from Cell Signaling and it has shown very good results.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates

Primary Incubation

4°C, overnight, 1:1000 in 5% BSA in TBST

Blocking Agent

5% milk in TBST

Secondary Incubation

Room temperature, 1h, 1:5000

Tertiary Incubation

NA

Detection

Bio-Rad claritiy

Results Summary

Very good antibody for p-AKT detection

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Perfect antibody for pAKT detection

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