Perfect pERK Antibody

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)

Catalog Number:

9101

I am investigating the role of the ERK-Pathway after extracellular vesicle treatment. I chose this product since I am also using the total Erk antibody from Cell Signaling and they are showing very good results.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates

Primary Incubation

4°C, overnight, 1:1000 in 5% BSA in TBST

Blocking Agent

5% milk in TBST

Secondary Incubation

1h room temperature, 1:5000

Tertiary Incubation

NA

Detection

Bio-Rad Clarity

Results Summary

Very good antibody for the detection of phospho Erk.

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use antibody.

The Bad

NA

The Bottom Line

Very good antibody for pERK detection

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