PD-1 Expression in Human Tissue

Internal Medicine.
Rush University Medical Center
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-human CD279 (PD-1)

Catalog Number:

329918

While we work on the mechanism of rheumatoid arthritis, we label PBMC and synovial fluid with CD3, CD4, PD-1, and I-Cos. The data show that PD-1 and I-Cos express higher in RA patient synovial fluid. This product was used to identify PD-1(CD279) in human tissue.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMC and synovial fluid.

Primary Incubation

1X10^6 PBMC or cells isolated from synovial fluid were incubated with 5ul anti-human CD279 (PD-1) Antibody PE/Cy7 and other antibodies in 30ul FACS buffer for 30 min at 4oC.

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This antibody shows great signal in cells from patient's synovial fluid.

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use.

The Bad

None

The Bottom Line

PD-1 expression is different in PBMC and synovial fluid.

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