Anti-Mouse CD86 BV510 for Splenocyte Staining

January 05, 2016

Tisch Cancer Institute
Icahn School of Medicine at Mount Sinai
Post-Doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 510™ anti-mouse CD86 Antibody

Catalog Number:

105040

Splenocytes were stained with CD86 to identify activated dendritic cells under various conditions (eg tumor bearing vs non tumor bearing mice)

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes, fixed with 2% PFA, permeabilized using Saponin buffer

Primary Incubation

1:400, 15min, 4C

Blocking Agent

Blocking Buffer containing sera (rat, rabbit, human, goat)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry (BD) LSRFortessa

Results Summary

Good signal to noise ratio, easy to distinguish between activated and resting cells

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bright fluorophore

The Bad

No complaints

The Bottom Line

Excellent antibody to identify activated DC

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