Qiagen, Gmbh, Germany
RNeasy® Mini Kit
74104
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Rat liver tissue was preserved in RNA later. For the RNA isolation and purification, liver tissue was homogenized and cells were lysed. RNA was purified in columns provided in the kit and collected for cDNA synthesis, which can be further applied in real-time PCR and northern blotting.
Real Time-PCR, Northern blotting
Rat liver tissue
The protocol was performed according to the manufacturer’s instructions. Liver tissue, which was stored in RNA later, was first homogenized and cells were lysed by lysis buffer. Silica membrane spin columns were used for isolation and purification of RNA. RNA present in the lysed sample will bind to the silica membrane of the column and the other contaminants are removed by washing. The purified RNA is eluted by RNase free elution water which dissolves the RNA and the eluent is collected.
None
The quality and purity of RNA was very good. The 260/280 ratio was always approximately 2.
100 µg of RNA from a sample is isolated and purified
RNA isolation and purification was very rapid with no chemical contamination and also requirement of starting material was also very less.
Qiagen RNeasy® Mini Kit offers efficient and rapid RNA isolation and purification.
Product Name: RNeasy Mini Kit (50)Supplier Page