A Quick and Easy to Isolate Mammalian RNA

Boston University
Pharmacology
Senior Post-doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

RNeasy mini kit (250)

Catalog Number:

74106

This was my first time trying this kit to isolate RNA from human HeLa cancer cell for real-time PCR. The manual is easy to follow and the quality of RNA isolated is great.

Experimental Design and Results Summary

Application

Isolation of mammalian RNA

Starting Material

HeLa cancer cell line

Protocol Overview

The protocol from the manufacturer is clear and easy to follow.

Tips

Monolayer cells can be lysed in the dish or wells directly. Use RNase free water and tips during the process of isolation.

Results Summary

Good quality of RNA is isolated and used for the following experiments. Everything work so easy and so well. Even a first-time user can isolate RNA without any problem.

Additional Notes

NA

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Summary

The Good

Easy, quick and good quality of RNA

The Bad

A little pricey

The Bottom Line

This kit makes isolation of RNA from mammalian cells easy and simple. It also facilitates the experiment since isolation is simplified.

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