Cell Signaling Technology
Phospho-p44/42 MAPK
9102S
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Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. We were testing this antibody to test activation of Erk in cancer cells via western blotting.
Western Blot
Cancer cells
1:3000, overnight at 4 C
5% milk
1:3000, one hour at room temperature
NA
ECL and film
As we expected Egf treatment increased Erk phosphorylation.
None
Very good antibody for western blot
Best antibody