Great Antibody for Ku70

Pharmacology
Boston University
Senior Post-doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

Ku70 antibody

Catalog Number:

GTX70271

Ku70 is a key protein involved in end joining of DNA double strand breaks. Using Western blot, the expression of Ku70 is determined. This Ku70 antibody from GeneTex is a mouse monoclonal antibody with very excellent sensitivity and specificity.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HeLa human cancer cell lysate

Primary Incubation

1:2000 in TBST

Blocking Agent

5% milk in TBST

Secondary Incubation

anti-mouse-HRP 1:5000

Tertiary Incubation

NA

Detection

Bio-Rad ECL and Bio-Rad ChemiDoc

Results Summary

A strong and specific band around 70kDa was observed. No background or non-specific bands were detected even after long exposure.

Additional Notes

None

Related Categories

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Summary

The Good

Specific, reliable and strong signal

The Bad

None

The Bottom Line

This is a high quality antibody and probably is the best Ku70 antibody in the market.

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