Cell Signaling ERK Antibody

December 30, 2015

Neurosurgery
Harvard Medical School
postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

ERK

Catalog Number:

4695

I am investigating the role of the ERK-Pathway after extracellular vesicle treatment. I chose this product since I am also using the phosphor-Erk antibody from Cell Signaling and they are showing very good results.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates

Primary Incubation

4°C overnight

Blocking Agent

5% Milk in TBST

Secondary Incubation

1h at room temperature

Tertiary Incubation

NA

Detection

Bio RAD

Results Summary

Very good ERK detection in all cell types used.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy handling, reliable results

The Bad

None

The Bottom Line

Perfect antibody to detect total ERK-Protein

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