Cell Signaling Technology
PIAS4 (D2F12) Rabbit mAb
4392
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PIAS4 of PIAS family is a SUMO E3 ligase. It has been implicated in many cellular processes including chromatin regulation and DNA repair. This antibody from CST specifically detects endogenous PIAS4 when used in western blotting, but failed to detect any positive signal in IF.
Western Blot
HeLa human cancer cell lysate
1:1000 in TBST
5% milk
Anti-rabbit-HRP 1:5000
NA
Bio-Rad ECL and Bio-Rad ChemiDoc
The antibody works well for western blotting. With specific siRNA against PIAS4, the antibody shows a nice specificity detecting endogenous PIAS4.
None
Specific and reliable
A bit weaker and needs longer incubation
Worth a try.