BD Biosciences
BV510 anti-mouse/human Ki67
563462
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Browse Similar Products: Ki-67 Flow Cytometry Antibodies
I used this antibody for staining Ki-67, a nuclear antigen that is expressed in cycling cells. The advantage is that you can stain cells and read them immediately instead of culturing them to see cell proliferation. Also beneficial for ex vivo staining of cells isolated from mice or human tissues/blood. The staining worked very well with the standard intra-cellular staining protocols.
Flow Cytometry
Purified mouse T cells
1:200 in Perm/Wash buffer
NA
Flow Cytometry - BV510 channel
The dot plots show a significant increase in fluorescence in antigen activated T cells, while the background in UN-stimulated cells was minimal.
None
Staining works well with minimal background from dead cells
Needs intracellular staining.
A good antibody for measuring cell proliferation during ex vivo experiments.