Portable Centrifuge Machine

National Dairy Research Institute
Animal Biochemistry
Scientist

Overall

Performance

Ease-of-Optimization

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Company:

Eppendorf

Product Name:

Centrifuge 5424

Catalog Number:

5424000.410

Blood samples collected in vaccutainers were placed in 1.5 ml Eppendorfs and centrifuged for plasma separation and also for protein and DNA isolation.

Experimental Design and Results Summary

Application

Centrifugation of blood samples for protein and DNA isolation. It is also used for the separation of plasma from whole blood.

Starting Material

Blood samples from rat

Protocol Overview

Samples to be centrifuged are placed in the centrifuge machine. Lid switch is present for opening and closing of centrifuge machine. Display is present to monitor the centrifugation time and speed. It has the capacity of 24 X 2 ml, and reaches a maximum of 21,130 xg. Samples can be centrifuged in microcentrifuge tubes (0.2 to 2 ml), PCR strips, spin columns (1.5 to 2 ml). Emergency lid release is also available.

Tips

None

Results Summary

Samples are centrifuged properly at adjustibe speed and duration. The pellets were tight and not dispersed. Plasma obtained as supernatant was clear and phase was separated properly.

Features Summary

Digital monitoring, adjustable speed and time.

Additional Notes

It is advisable not to use the centrifuge near reactive chemicals, explosives and should not be used for centrifuging highly reactive substances

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Summary

The Good

User friendly

The Bad

Temperature can’t be adjusted

The Bottom Line

Easily operatable centrifuge machine.

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