Flow Cytometry Antibody with Good Signal but Some Leakage

December 21, 2015

Internal Medicine
University of California
Assistant Project Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD11c-PEcy7 conjugate

Catalog Number:

117317

CD11c distribution was used to study osteoclast precursor subsets in the spleen and bone marrow.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:1000 in Fc block

Blocking Agent

Fc Block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

PE-Cy7 on Aria

Results Summary

Specific antibody but PE-Cy7 does bleed in PE channel, needs proper isotype and gating to visualize correctly.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Specificity

The Bad

Bleeds in PE channel

The Bottom Line

Overall good if proper gating and isotype is used. Most importantly, I recommend using a high dilution.

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