Boster
PLK2 antibody
PA2131
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I wanted to investigate the expression of PLK2 protein after ShRNA-Plk2 treatment in UT7/Epo-S1 cell. Western blot was applied to test the expression of PLK2 with the antibody from Boster (PA2131, small volume). I chose this antibody because of the small volume and it claimed to work on western blot.
Western Blot
Cell lysis sample
1:1000 dilution for incubation 1.5h at room temprature
5% non-fat milk
Anti-Rabbit HRP
1:10,000 dilution for incubation 1h at room temprature
ECL method
A lot of unspecific bands.
None
We exclude an antibody
We need to try another one
This antibody may not be good for western blot in UT7/Epo-S1 cell and 293 cells.