Avidin/Biotin Blocking Kit

Miami
MI
Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Avidin/Biotin Blocking Kit

Catalog Number:

00-4303

This is used to minimize background from avidin binding to proteins in the tissue or sample. This is a good kit to use if one has biotin labeled antibodies and will be using strep. secondaries.

Experimental Design and Results Summary

Application

Immunofluorescence

Starting Material

None

Protocol Overview

Add solution (A) and incubate for 20 min. then wash and add solution (B) for 20 min. Wash after solution B. Sample

Tips

Add enough to immerse the sample

Results Summary

Immunofluorescent samples with biotinylated primaries have low background when using this kit.

Additional Notes

None

Related Categories

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Summary

The Good

Minimizes background

The Bad

None

The Bottom Line

This is an easy to use kit for inhibiting background

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