Anti-Mouse CD4 PE for Splenocyte Staining

Tisch Cancer Institute
Icahn School of Medicine at Mount Sinai
Post-Doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD4

Catalog Number:

100512

This antibody was used to identify CD4 T cells in mouse spleen in order to analyze overall cell numbers and subset differentiation under different conditions (tumor bearing vs non tumor bearing mice).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:400, 15min, 4C

Blocking Agent

Blocking Buffer containing sera (rat, rabbit, human, goat)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry (BD) LSRFortessa

Results Summary

This antibody has an excellent signal to noise ratio for mouse splenocytes, making it very easy to identify CD4 T cells and subject them to further analysis.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bright fluorophore

The Bad

None

The Bottom Line

Excellent antibody for identification of CD4 T cells.

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