Western Blot Using Anti-Nephrin Antibody from Abcam

We performed protein expression analyses in human podocytes regarding the generation of different factors involved in apoptosis regulation, signaling, cell structure maintenance and cell viability. Specifically phospho-specific antibodies were used to analyse the signaling activity normalized againt total protein expression (see Akt, AMPK and mTOR). We performed Western blot analyses of nephrin protein expression in cell lysates of human podocytes using the anti-Nephrin antibody from Abcam. We detected nephrin.