Cell Signaling Technology
Caspase-3 (3G2) Mouse mAb
9668S
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We performed protein expression analyses in human podocytes regarding the generation of different factors involved in apoptosis regulation, signaling, cell structure maintenance and cell viability. Specifically phospho-specific antibodies were used to analyse the signaling activity normalized againt total protein expression (see Akt, AMPK and mTOR). With this antibody, we performed Western blot analyses of Caspase-3 in cell lysates of human podocytes using the Caspase-3 (3G2) mouse mAb from Cell Signaling Technology.
Western Blot
Cell lysate of human podocytes
Dilution 1:500, 16 hours, 4°C
5% Skim milk in TBST buffer
Dilution: 1:5000, 1 hour, 24°C
ECL - 5 min, room temperature
ECL
A single band was detected after 5 min (Caspase3-band at 35 kDa; see figure).
None
No cleaved Caspase 3 was detected in our experiments
This antibody is ok for Western blot analyses of total caspase 3 in human podocytes