Oxphos Antibody for Human

Environ. Med.
University of Rochester
Postdoc

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Quality of Results

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Company:

Abcam

Product Name:

Total OXPHOS Human WB Antibody Cocktail (ab110411)

Catalog Number:

ab110411

We have tested this antibody in human fibroblast cells after exposing the cells to oxidants.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human fibroblast cells

Primary Incubation

1:1000 in 5% milk

Blocking Agent

5% milk at RT for 1 hr

Secondary Incubation

1:10,000 dilution HRP-conjugated anti-mouse IgG

Tertiary Incubation

NA

Detection

ECL based detection

Results Summary

We were able to detect only 3-4 different complexes using this antibody in human cell lysates

Additional Notes

None

Related Categories

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Summary

The Good

Works for human cell lysates

The Bad

Noted none

The Bottom Line

Works in human cell lysates not tested in mouse samples.

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