Warner Instruments
Recording chamber for tissue slice, with SHD-22L/15 anchor
64-0228
We utilize in vitro whole-cell patch-clamp recordings to examine the characteristics of the maturation of GABAergic transmission and factors that may disrupt this maturation, within the context of disease states. We picked the Warner Instruments recording chamber for its versatility, ease of installation/maintenance, and quality construction.
Whole-cell patch-clamp recordings
None
The chamber is integrated with out patch-clamp rig in that it holds our brain slices stably during the course of recordings, while integrating effective perfusion and evacuation of artificial cerebrospinal fluid/drugs, temperature controls, and electrical grounding.
Make sure that you thoroughly clean your chamber after each use. If left unchecked, the buildup of aCSF salts can be a significant source of noise.
We used this piece of equipment to collect data on spontaneous and miniature inhibitory postsynaptic currents (IPSC), evoked IPSCs, E/I ratios, and a wide variety of pharmacological manipulations.
The customizable nature of the chamber allows for easy day-to-day adjustments to suction and fluid level, easy cleaning, and easy integration into existing recording systems.
Make sure you stay aware of the state of your coverslip. They have a tendency to fracture, creating a small leak that facilitates salt buildup. Easy to fix, but potentially a large problem if unaddressed.
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Good construction, extremely versatile in its applications
Salt builup without meticulous, regular maintenance
If you take care of it and maintain your rig, this is a great product, well worth the purchase.