APC Anti-Mouse/Human IL-5 Antibody

December 03, 2015

Medicine
Brigham and Women's Hospital
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse/human IL-5 Antibody

Catalog Number:

504305

The purpose of this study was to identify IL-5 producing lymphocytes in the mediastinal lymph node after allergen sensitization in mice. The product was recommended by a colleague.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Lymph node cells

Primary Incubation

1:200, in PBS on ice for 30 min

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometer

Results Summary

Clearly identified IL-5 producing cells and allowed for comparison between mice with different genetic background.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Clean antibody

The Bad

None

The Bottom Line

Highly recommended

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