Great Antibody for Phenotyping of 1C6 Mice

Neurology
BWH
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse TCR Vb7

Catalog Number:

118308

Our lab is interested in central and peripheral tolerance in the context of autoimmunity with a particular interest in multiple sclerosis. 1C6. IgH NOD mice are transgenic NOD mice expressing TCR and BCR for the autoantigen MOG. These mice develop spontaneous EAE (a mouse model of multiple sclerosis)

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Blood cells from 1C6.IgH NOD mouse

Primary Incubation

20min 4'C, dilution 1/200 (50ul)

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS LSRII

Results Summary

Bright and specific staining for the transgenic TCR Vb7, identifying the mice as 1C6 (bearing the TCR for MOG). In 1C6 mice, both CD4+ and CD8+ T cells bear the transgenic T cell receptor

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bright and specific staining

The Bad

None

The Bottom Line

Perfect antibody for phenotyping of 1C6 mice (bearing the transgenic TCR for MOG)

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