Perfect Antibody for Detection of T Cells

November 06, 2015

Neurology
BWH
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD3e

Catalog Number:

100312

Our laboratory is interested in the role of T cells in autoimmune diseases. CD3 is a common marker for T cells: TCRalpha/beta+ and TCRgamma/delta+ T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen cells

Primary Incubation

20min, 4'C, dilution: 1/200 (50ul)

Blocking Agent

Fc block (anti CD16/CD32)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS LSRII

Results Summary

Very bright staining and clear separation of CD3+ positive and negative T cells.

Additional Notes

It is important to stain at 4'C as CD3 is rapidly internalized after antibody binding at room temperature or higher (antigenic modulation)

Related Categories

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Summary

The Good

Bright staining

The Bad

Nothing bad found

The Bottom Line

Excellent antibody for detection of T cells

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