Sirt7 Expression in Neuronal Cells

December 02, 2015

Project JRF
Centre for Cellular & Molecular Biology
Postdoctoral Researcher

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Company:

Abcam

Product Name:

Anti-SIRT7 antibody

Catalog Number:

ab78977

This product was used to check Sirt7 expression in neuronal cells in different experimental conditions. The product is standardised for western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Neuro2A cell lysate

Primary Incubation

1:500(1ug/mL) in 5%BSA-1XTbst, 4C, overnight

Blocking Agent

5%BSA-1XTbst, 1h, Room temperature

Secondary Incubation

Anti-rabbit, 1:5000 in 5%BSA-1XTbst, Room temperature, 2h

Tertiary Incubation

No tertiary incubation

Detection

Luminol-chem doc instrument

Results Summary

Sirt7 expression at protein level is changing in Neuronal cells(N2A).

Additional Notes

None

Related Categories

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Summary

The Good

Antibody is specific to Sirt7

The Bad

High protein need to be loaded

The Bottom Line

Recommended for western blot.

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