Novus Biological
eIF2A [p Ser51] Antibody
NBP1-51469
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The general aim of this project was to assess total protein translation attentuation in a variety of treatment groups using total and phosphorylated eIF2alpha, a downstream component of PERK. To do this we compared phosphorylated eIF2alpha to total using western blotting.
Western Blot
Total cell lysates of H4IIE rat hepatoma cells
1:500 antibody, 0.1% tween, in 1:3 li-cor odyssey block: TBS overnight at 4 degrees C.
Li-Cor Odyssey Block for 1 hour.
1:15,000 Li-Cor IRDye 800CW, 0.1% tween, 0.02% SDS, in 1:3 odyssey block: TBS at room temperature for 1 hour
NA
Li-Cor Odyssey infrared Imaging system
Phosphorylated eIF2alpha indicates translation attentuation. This antibody detects endogenous levels of eIF2alpha protein when phosphorylated at S51.
None
Works well, clearly visible.
This antibody works well and has little background signal. Followed antibody directions and did not have to optimize. Worked on the first try!