Santa Cruz Biotechnology, Inc.
eIF2a (D-3)
sc-133132
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The purpose of this study was to assess a mechanism responsible for possible protein synthesis in a variety of treatments. Phosphorylated eIF2a is responsible for global protein translation attenuation in the ER and is activated in response to ER stress and UPR activation. The goal of this experiment was to assess the amount of phosphorylated eIF2a to be able to determine how much protein synthesis is occurring or not occurring in a variety of treatment groups.
Western Blot
Total cell lysates of H4IIE rat hepatoma cells
1:1000 antibody, 0.1% tween, in 1:3 li-cor odyssey block: TBS overnight at 4 degrees C.
Li-Cor Odyssey Block for 1 hour.
1:15,000 Li-Cor IRDye 680RD, 0.1% tween, 0.02% SDS, in 1:3 odyssey block: TBS at room temperature for 1 hour
NA
Li-Cor Odyssey infrared Imaging system
Total eIF2a is clearly visible and has little background signal.
None
Clearly visible. Little to no background signal.
Total eIF2a antibody works well and no optimization was required.