Abcam
Goat F(ab')2 Anti-Human IgG (Fab')2 (HRP)
ab98535
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This antibody is specific to Fab; it does not react with Fc. This was tested in western blotting loaded with human IgG and human Fc-fusion protein without any incubation with primary antibody.
Western Blot
Human IgG and Fc-fusion protein
No primary antibody used
5% milk in TBS buffer 1 hour at room temperature
1:3000 in TBST buffer 1 hour at room temperature
NA
ECL incubated at room temperature for 1 min
None
Specific to Fab, it does not react with Fc
Not found so far.
This antibody will be quite useful in ELISA and western blotting where a specific Fab secondary is needed.