HIF-2alpha Antibody NB100-132 from Novus Biologicals

December 11, 2015

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

HIF-2alpha Antibody

Catalog Number:

NB100-132

This antibody was used to detect HIF-2alpha expression in response to ionized radiation treatment in HN5 human head and neck cancer cells by western blot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human HNSCC cell line whole cell lysate

Primary Incubation

1:1000, overnight at 4 degrees

Blocking Agent

5% milk

Secondary Incubation

1:2000, 1 hour at RT

Tertiary Incubation

NA

Detection

Enhanced Chemiluminescence Detection Kit

Results Summary

This antibody detected HIF-2alpha expression in HN5 cells, but with a lot of non-specific bands and dirty background.

Additional Notes

None

Related Categories

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Summary

The Good

Detects specific band easily

The Bad

Many non-specific bands; cannnot be re-used.

The Bottom Line

This antibody can be used to detect HIF-2alpha expression by western blot in HN5 cells. However, when diluted, this antibody cannot be recycled and re-used.

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