Novus Biologicals
p-FoxO3 (S253) Antibody
NBP1-19927
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This antibody was used to detect expression of phospho-FoxO3 (S253) in response to U0126 treatment in MDA-MB-231 human breast cancer cells by western blot.
Western Blot
Human breast cancer cell line whole cell lysate
1:1000, overnight at 4 degrees
5% milk
1:2000, 1 hour at RT
NA
ECL Prime
This antibody detected the expression of phospho-FoxO3 at S253 site in MDA-MB-231 cells.
None
Detects specific band with clean background
Signal is weak
This antibody worked to detect the expression of phospho-FoxO3 at S253 site, although the signal is weak.