Phospho-p53 (Ser15) Antibody from Cell Signaling Technology

December 07, 2015

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

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Company:

Cell Signaling Technology

Product Name:

Phospho-p53 (Ser15) Antibody

Catalog Number:

9286

This antibody was used to detect expression of phospho-p53 (Ser15) in response to ionized radiation treatment in HN5 human HNSCC cells by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human HNSCC cell line whole cell lysate

Primary Incubation

1:1000, overnight at 4 degrees

Blocking Agent

5% milk

Secondary Incubation

1:2000, 1 hour at RT

Tertiary Incubation

None

Detection

Enhanced Chemiluminescence Detection Kit

Results Summary

This antibody detected phospho-p53 at Ser15 site with some non-specific band, which did not affect results.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Detects specific bands and the signal is strong

The Bad

Has some non-specific bands and dirty background

The Bottom Line

This antibody successfully detected phospho-p53 at Ser15 site. Although there are some non-specific bands, they did not affect the experiment results.

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