Phospho-EGFR (Tyr845) Antibody from Cell Signaling

December 04, 2015

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-EGFR (Tyr845) Antibody

Catalog Number:

6963

This antibody was used to detect the expression of phosphorylated EGFR at Tyr845 site in response to cetuximab treatment in HN5 human HNSCC cells by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human HNSCC cell line whole cell lysate

Primary Incubation

1:2000, overnight at 4 degrees

Blocking Agent

5% milk

Secondary Incubation

1:2000, 1 hour at RT

Tertiary Incubation

NA

Detection

Enhanced Chemiluminescence Detection Kit

Results Summary

This antibody detected the expression of phospho-EGFR (Tyr845) easily with clean back ground.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Detects specific band easily

The Bad

None

The Bottom Line

This is a perfect antibody for detection of phospho-EGFR (Tyr845) by western blot.

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