Thermo Fisher (Applied Biosystems)
Primer/probe set for beta-glucuronidase
4326320E
We work with human sinus tissue and usual housekeeping genes for RT-PCR, like GAPDH, were found to have different baseline expression in nasal polyps compared to uncinate tissue, which could confound results. We found that beta-glucuronidase, or bGUS, had the most consistent expression in nasal polyps and uncinate tissue.
RT-PCR
Human sinus tissue lysates
The primer/probe set is a 20x solution, so it you're using 20uL reactions you only need 1uL/reaction.
This is available on VIC, so you can multiplex with FAM primer probe sets to save sample and reagents
bGUS is the most reliable housekeeping gene for human sinus tissue.
Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease. Pothoven KL, Norton JE, Hulse KE, Suh LA, Carter RG, Rocci E, Harris KE, Shintani-Smith S, Conley DB, Chandra RK, Liu MC, Kato A, Gonsalves N, Grammer LC 3rd, Peters AT, Kern RC, Bryce PJ, Tan BK, Schleimer RP. J Allergy Clin Immunol. 2015 Sep;136(3):737-746.e4. doi: 10.1016/j.jaci.2015.01.043.
Great primer/probe set, super easy
More expensive than sequencing primers and probes
This is the best housekeeping gene for human sinus tissue, and multiplexing with other genes saves reagents and sample.