Fitzgerald Industries International
RSV antibody (FITC)
60-R41
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Browse Similar Products: RSV Flow Cytometry Antibodies
This product was used to detect RSV infected PBMC by flow cytometry with single step staining.
Flow Cytometry
Fresh human PBMC
45 minutes in the dark at ambient temperature
PBS supplemented with 0,5% bovine serum albumin
NA
Fixation with 3.7% Formalin in PBS supplemented with 1% bovine calf serum
BD FACS Canto II with FACS diva software
It took a few experiments to get this antibody working, because it produces high background on uninfected cells. Cells with low expression of antigen or early during infection can be missed due to high background. Infection of RSV was verified by two step stainings using alternative antibodies. With this FITC-conjugated antibody, we detect less positive cells than expected. Uninfected control cells are always necessary due to the high background. The antibody works better on homogeneous cell populations like immortalized cell lines.
Graph shows antibody dilution series
High concentrations of antibody gives very robust signal; cheap product
High background staining.
The signal to noise ratio is not optimal.