Migration and Wound Healing Assays

Protocol Overview

This chamber is used for wound healing assays. Inside the chamber the cell suspension were loaded on both the wells and cells were allowed to grow. After the cells got attached wells were slowly removed using sterile tweezers. The adherent cells were grown only in the place were the wells were placed. The cells were then washed with PBS once followed by the treatment then migrated cells were observed under microscopy at various timepoints.