HIF-1alpha Antibody from BD Transduction Laboratories

December 01, 2015

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

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Company:

BD Transduction Laboratories

Product Name:

HIF-1alpha Antibody

Catalog Number:

610958

Info: View Product View Product Specs

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This antibody was used to detect the expression of the alpha subunit of HIF-1 in response to cetuximab treatment in human HNSCC cells under hypoxic conditions by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human HNSCC cell line whole cell lysate

Primary Incubation

1:500, overnight at 4 degrees

Blocking Agent

5% milk

Secondary Incubation

1:2000, 1 hour at RT

Tertiary Incubation

NA

Detection

Enhanced Chemiluminescence Detection Kit

Results Summary

This antibody detected changes of the alpha subunit of HIF-1 protein in human HNSCC cell lines.

Additional Notes

None

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Summary

The Good

Can detect specific bands under hypoxic condition

The Bad

Has some non-specific bands; after diluted, this antibody can be used for only a couple of times

The Bottom Line

This antibody works to detect HIF-1alpha, but it is better to use fresh antibody since it becomes pretty weak after a couple of re-uses.

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