ERK1/2 Antibody from Cell Signaling Technology

November 13, 2015

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

ERK1/2

Catalog Number:

9102

This antibody was used to detect total expression of p44/42 MAPK (ERK1/2) in response to cetuximab treatment in human HNSCC cells by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human HNSCC cell line whole cell lysate

Primary Incubation

1:2000, 2 hours at RT

Blocking Agent

5% milk

Secondary Incubation

1:2000, 1 hour at RT

Tertiary Incubation

NA

Detection

Enhanced Chemiluminescence Detection Kit

Results Summary

This antibody detects total ERK1/2 and is used as a control for phosphorelated ERK.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Detects a specific band and the signal is strong

The Bad

None

The Bottom Line

This is a perfect antibody for western blot.

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