Great Antibody for Intracellular Staining

November 03, 2015

Microbiology
Medical College of Wisconsin
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-mouse IFN-γ Antibody

Catalog Number:

505825

Info: View Product View Product Specs

Browse Similar Products: IFN-gamma Antibodies

This antibody was recommended by a collaborating lab for the detection of IFNg-producing CD8+ T cells after restimulation with non-specific agents or viral peptide.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse PECs

Primary Incubation

25 min

Blocking Agent

FC block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

LSRII Flow Cytometer

Results Summary

Works well with our permeabilization kit. Able to detect > 50% CD8+ cells producing IFNg after non-specific restimulation.

Additional Notes

None

Related Categories

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Summary

The Good

Works well

The Bad

None

The Bottom Line

Buy this antibody if you need to detect IFNg producing Tcells

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